CENP-C Is a Structural Platform for Kinetochore Assembly
作者： Marcin R. Przewloka , Zsolt Venkei , Victor M. Bolanos-Garcia , Janusz Debski , Michal Dadlez
DOI: 10.1016/J.CUB.2011.02.005
关键词:
摘要: Centromeres provide a region of chromatin upon which kinetochores are assembled in mitosis. Centromeric protein C (CENP-C) is core component this centromeric that, when depleted, prevents the proper formation both centromeres and kinetochores. CENP-C localizes to throughout cell cycle via its C-terminal part, whereas N-terminal part appears necessary for recruitment some but not all components Mis12 complex kinetochore. We now find that kinetochore proteins belonging KMN (KNL1/Spc105, complex, Ndc80 complex) network bind Drosophila CENP-C. Moreover, we show Nnf1 interacts directly with vitro. To test whether CENP-C's was sufficient recruit proteins, targeted it centrosome by fusing domain Plk4 kinase. The complexes Spc105 were then recruited centrosomes at expense centromeres, leading mitotic abnormalities typical cells defective Thus, acts as principal linkage between centromere during
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