Ultrasound pretreatment enhances the inhibitory effects of nisin/carvacrol against germination, outgrowth and vegetative growth of spores of Bacillus subtilis ATCC6633 in laboratory medium and milk: Population and single-cell analysis.
作者： Lihua Fan , Balarabe Bilyaminu Ismail , Furong Hou , Aliyu Idris Muhammad , Tian Ding
DOI: 10.1016/J.IJFOODMICRO.2019.108329
关键词:
摘要: This study evaluated the synergetic inhibitory effects of ultrasound and nisin/carvacrol on spore germination, outgrowth, subsequent growth vegetative cell Bacillus subtilis in laboratory medium milk. Ultrasound pretreatment (3.33 W/mL, 15 min) (0.01%, 0.02%) synergistically inhibited spores medium. Whereas no such effect was observed milk even with a 10-fold increase concentration (1%) nisin. Flow cytometry analysis showed that germination capacities pretreated combined (67.3% 30.5%, respectively) lower than untreated (95.1%). These results quantitatively revealed treatments which were confirmed by phase-bright observations at single level. In general, current work identified ultrasound-carvacrol treatment as an effective strategy to control cells B. during abusive storage.
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