Two monoclonal antibody lines directed against Subunit IV of cytochrome oxidase: A study of opposite effects☆

作者: Wen-Zhi Gai , Shu-Man Sun , You-Zhan Ding , Jo A. Freedman , Samuel H.P. Chan

DOI: 10.1016/0003-9861(88)90296-2

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摘要: Two monoclonal lines of antibodies were isolated with specificities against the amino half Subunit IV beef heart cytochrome oxidase. The had nonoverlapping epitopes. Both bound to matrix face membranous oxidase, neither cytoplasmic face. One line (QA4/C4) stimulated electron transfer in soluble or while other (QA4) inhibited that activity by both oxidase preparations. These effects on not altered inclusion omission detergent. ATP depressed binding either antibody In absence ATP, QA4/C4 only high affinity phase c oxidation (with decreased KM and increased Vmax), causing slight inhibition low KM). presence abolished phase, but did alter influence phase. QA4 leaving a similar induced ATP. seen alone. This behavior is consistent dissection two catalytic sites for more than one affector site.

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