Contact-mediated reversible suppression of myogenesis

摘要: Abstract Myogenic cells isolated from the breast muscles of 11-day chick embryos by trypsinization proliferate and fuse to form multinucleated muscle fibers. When such were plated onto confluent, nonproliferating layers heterotypic or homotypic cells, no formed. Autoradiography, using thymidine-3H labeled myogenic demonstrated that attached cellular substrates, remained attached, but did not divide. which had been kept forming on substrates could be removed subcultured muscle. Muscle formed when they cultured a millipore filter placed over confluent substrate. also in “hole” cut culture appear tissue surrounding “hole.” Populations large fraction capable fusing without undergoing mitosis indicating interfere directly with processes fusion. Cellular fixed absolute alcohol irradiated ultraviolet light permitted myogenesis their surfaces. Glutaraldehyde-fixed however, prevented myogenesis. It is suggested suppression differentiation result cell-cell cell-interstitial matrix contacts primarily inhibit precursor cells.