The synthesis of polyamide-oligonucleotide conjugate molecules
作者： Jim Haralambidis , Lucy Duncan , Karin Angus , Geoffrey W. Tregear
DOI: 10.1093/NAR/18.3.493
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摘要: We have developed methods for the synthesis of peptide-oligodeoxyribonucleotide conjugate molecules in particular, and polyamide-oligonucleotide conjugates general. Synthesis is carried out by a solid-phase procedure involves assembly polyamide on solid support, conversion terminal amino group to protected primary aliphatic hydroxy reaction with alpha, omega-hydroxycarboxylic acid derivatives, finally oligonucleotide using phosphoramidite chemistry. The can be used as DNA probes, component carrying one or more non-radioactive markers. These also potential anti-sense inhibitors gene expression, peptide segment acting targeting moiety.
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