作者: John J. Kilbane , Benjamin Stark
DOI: 10.1007/S11274-016-2084-6
关键词:
摘要: Biological desulfurization (biodesulfurization) of dibenzothiophene (DBT) by the 4S pathway is a model system for an enviromentally benign way to lower sulfur content petroleum. Despite large amount effort efficiency still too low commercial oil biodesulfurization process, but could potentially be used now processes produce surfactants, antibiotics, polythioesters and other chemicals detoxification some chemical warfare agents. Proteins containing disulfide bonds are resistant temperature, pH, solvents, production disulfide-rich proteins in microbial hosts challenging. The study can provide insights as how maximize proteins. Engineering operon encoding contain greater methionine cysteine may able link use DBT sole source increasing activity nutritional demand sulfur. This strategy result development biocatalysts suitable also lead better understanding physiology optimize mult-step co-factor-requiring pathway, well highly stable industrially relevant enzymes numerous applications.