A novel colorimetric assay for the determination of lysophosphatidic acid in plasma using an enzymatic cycling method

摘要: Abstract Background : Several methods for measuring concentrations of lysophosphatidic acid (LPA), a lipid mediator, have been reported to date. However, these are not routinely used because most them require specialized instrument and complicated protocol. Methods We developed novel LPA assay using enzymatic cycling. in sample is hydrolyzed with lysophospholipase glycerol-3-phosphate, followed by cycling glycerol-3-phosphate oxidase dehydrogenase. Amplified hydrogen peroxides, product the cycling, then colorimetrically measured. Results This method was specific LPA, being insensitive presence phosphatidic or lysophosphatidylcholine. The within-run between-run CVs were 1.31–1.32% 0.73–1.03%, respectively. recoveries exogenous added plasma 100.3–101.6%. In males, (mean±S.D.) human serum EDTA-plasma 0.41±0.14 0.08±0.02 μmol/l, females, they 0.41±0.12 0.09±0.02 Conclusions colorimetric determination simple highly sensitive. It can be an automatic analyzer. may also useful further studies biological functions as well clinical applications various disorders.