Method and reagent for inhibiting the expression of disease related genes

摘要: Enzymatic RNA molecules which cleave ICAM-1 mRNA, IL-5 rel A TNF-α RSV mRNA or genomic RNA, CML associated and use of these for the treatment pathological conditions related to those mRNA-levels; ribonucleosides nucleotides modified in 2', 3' 5', methods their synthesis, purification deprotection; vectors containing multiple enzymatic nucleic acids, optionally chimeric form with tRNAs; method introducing acids into cells by forming a complex second acid, where is capable taking an R-loop base-paired structure; altering mutant acid vivo hybridization oligonucleotide activating dsRNA deaminase, comprising activity chemical mutagen. Further are disclosed trans-cleaving -ligating hairpin ribozymes lacking substrate moiety, as well hammerhead having interconnecting loop between base pairs stem II.