A new HPLC method for serum neopterin measurement and relationships with plasma thiols levels in healthy subjects
作者： Ciriaco Carru , Angelo Zinellu , Salvatore Sotgia , Roberta Serra , Maria Franca Usai
DOI: 10.1002/BMC.325
关键词:
摘要: Neopterin, a pyrazinopyrimidine compound, serves as marker of cellular immune system activation, and it can be used prognostic predictor for certain types diseases. We propose new simple HPLC method to measure serum neopterin with highly sensitive fluorimetric detection. After TCA protein precipitation, the supernatant was diluted five times, injected into C18 reversed-phase column eluted at flow rate 1.5 mL/min by an isocratic water-acetonitrile (99:1) mobile phase. The natural fluorescence molecule detected excitation wavelength 353 nm emission 438 nm. In these conditions retention time about 4 min. Our proposed compared validated chromatographic separation, obtained data from 35 healthy volunteers were analysed Passing-Bablok regression Bland-Altman test. Neopterin measurement in subjects also employed investigate on its potential relationships plasma thiols levels.
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sci-hub.se 参考文章(21)
B. Widner, N. Sepp, E. Kowald, S. Kind, M. Schmuth, D. Fuchs, Degradation of Tryptophan in Patients with Systemic Lupus Erythematosus Advances in Experimental Medicine and Biology. ,vol. 467, pp. 571- 577 ,(1999) , 10.1007/978-1-4615-4709-9_71
Christian Murr, Anton Bergant, Martin Widschwendter, Kurt Heim, Hans Schröcksnadel, Dietmar Fuchs, Neopterin Is an Independent Prognostic Variable in Females with Breast Cancer Clinical Chemistry. ,vol. 45, pp. 1998- 2004 ,(1999) , 10.1093/CLINCHEM/45.11.1998
Mistunori Ikeda, Yasuaki Hirose, Ken Miyoshi, Hajime Kodama, Nuclear factor κB (NF-κB) activation by hydrogen peroxide in human epidermal keratinocytes and the restorative effect of interleukin-10 Journal of Dermatological Science. ,vol. 28, pp. 159- 170 ,(2002) , 10.1016/S0923-1811(01)00164-5
Setsuko Katoh, Terumi Sueoka, Sadao Matsuura, Takashi Sugimoto, Biopterin and neopterin in human saliva Life Sciences. ,vol. 45, pp. 2561- 2568 ,(1989) , 10.1016/0024-3205(89)90240-3
G. Saez, P.J. Thornalley, H.A.O. Hill, R. Hems, J.V. Bannister, The production of free radicals during the autoxidation of cysteine and their effect on isolated rat hepatocytes Biochimica et Biophysica Acta. ,vol. 719, pp. 24- 31 ,(1982) , 10.1016/0304-4165(82)90302-6
Irene Wede, Bernhard Widner, Dietmar Fuchs, Neopterin derivatives modulate toxicity of reactive species on Escherichia coli Free Radical Research. ,vol. 31, pp. 381- 388 ,(1999) , 10.1080/10715769900300951
D. Fuchs, A. Hausen, M. Kofler, H. Kosanowski, G. Reibnegger, H. Wachter, Neopterin as an index of immune response in patients with tuberculosis. Lung. ,vol. 162, pp. 337- 346 ,(1984) , 10.1007/BF02715666
Bernhard Widner, Gabriele Baier-Bitterlich, Irene Wede, Barbara Wirleitner, Dietmar Fuchs, Neopterin Derivatives Modulate the Nitration of Tyrosine by Peroxynitrite Biochemical and Biophysical Research Communications. ,vol. 248, pp. 341- 346 ,(1998) , 10.1006/BBRC.1998.8856
Peter Kotanko, Raimund Margreiter, Walter Pfaller, Urinary N-acetyl-β-D-glucosaminidase and neopterin aid in the diagnosis of rejection and acute tubular necrosis in initially nonfunctioning kidney grafts Nephron. ,vol. 84, pp. 228- 235 ,(2000) , 10.1159/000045582
D. E. Griffin, J. C. McArthur, D. R. Cornblath, Neopterin and interferon‐gamma in serum and cerebrospinal fluid of patients with HIV‐associated neurologic disease Neurology. ,vol. 41, pp. 69- 74 ,(1991) , 10.1212/WNL.41.1.69