Spin∞ an improved miniaturized spinning bioreactor for the generation of human cerebral organoids from pluripotent stem cells
作者： Alejandra I. Romero-Morales , Brian J. O’Grady , Kylie M. Balotin , Leon M. Bellan , Ethan S. Lippmann
DOI: 10.1101/687095
关键词:
摘要: Three-dimensional (3D) brain organoids derived from human pluripotent stem cells (hPSCs), including embryonic (hESCs) and induced (iPSCs), have become a powerful system to study early development events model disease. Cerebral are generally produced in static culture or vessel with active mixing, the two most widely used systems for mixing large spinning flask miniaturized multi-well bioreactor (also known as Spin Omega (SpinΩ). The SpinΩ provides that is amenable drug testing, has increased throughput reproducibility, utilizes less media. However, technical limitations of this include poor stability select components an elevated risk contamination due inability sterilize device preassembled. Here, we report new design system, which term Spinfinity (Spin∞) overcomes these concerns permit long-term experiments.
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