A radioprotective effect of imatinib (Gleevec) in human squamous carcinoma cells.

摘要: Purpose: To study the radiation response-modifying effect of imatinib (Gleevec®) in a squamous cell carcinoma line, PECA. Material and Methods: Cytotoxicity was determined by colony forming multiplying capacity. Drug stability shown HPLC. Multidrug resistance phenotype studied rhodamine-123 efflux. Cell-cycle responses were measured flow cytometry. Homologous recombination repair Rad51 immunohistochemistry. Results: Inactivating 50% PECA cells required approximately 7 µM imatinib. The drug did not decay nor it degraded during test periods. efflux occurred only to minor extent. Multiplying capacity but survival fractions revealed radioprotective There cell-cycle alterations presence rate Rad51-positive foci significantly increased. Conclusion: apparently lack highly specific target for In surviving at high concentrations, may exert on inducing DNA repair. Under prolonged exposure, drug-resistant show an accelerated recovery from acute or delayed damage.