Classification of organelle trajectories using region-based curve analysis

摘要: A method based on analysis of the region movement and functioning acto-myosin cytoskeleton has been elaborated to quantify classify patterns organelle in tobacco pollen tubes. The trajectory was dilated movement, which then reduced give a one-pixel-wide skeleton, represented by graph structure. longest line this skeleton hypothesized represent basic track along single actin filament. Quantitative features were derived from structure, direction skeletal line, distance between particle. These corresponded biological events like amount linear or probability attachment an From 81 analyzed trajectories, 17 had completely linear, nonlinear, 47 alternating non-linear movement. Selected employed for classification ranking representative sample population organelles moving cell tip. presented methods can be applied any field where particle motion are intended. Cytometry 29:136‐146, 1997. r 1997 Wiley-Liss, Inc.