Sensitive non-isotopic DNA hybridisation assay or immediate-early antigen detection for rapid identification of human cytomegalovirus in urine.
作者： Colin P. Kimpton , David J. Morris , Gerald Corbitt
DOI: 10.1016/0166-0934(91)90188-6
关键词:
摘要: A sensitive non-radioactive DNA hybridisation assay employing digoxigenin-labelled probes was compared with immediate-early antigen detection and conventional virus isolation for the identification of human cytomegalovirus (HCMV) in 249 urine samples. Of 44 specimens yielding HCMV by isolation, more were positive (32; 73%) than (25; 52%) (P = 0.05). The specificity 45 apparently falsely supported 40 these using polymerase chain reaction. Many may thus contain large amounts non-viable or free viral DNA. Evaluation various protocols extraction denaturation prior to showed that proteinase K digestion phenol/chloroform most reliable procedure. We conclude described is a potentially valuable routine diagnostic test.
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