Cellular localization and effect of nitric oxide synthesis in a rat model of orthotopic liver transplantation.

摘要: Nitric oxide (NO) is a multifunctional free radical with variety of described biochemical and physiological roles. The immunologic relationships between organ transplantation NO synthesis are unknown. While number in vitro vivo models have demonstrated an immunomodulatory role for NO, results suggest both immunosuppressive immunostimulatory inaction. In order to better delineate the liver transplantation, Kamada model rat OLT strain combinations simulating acute rejection spontaneous hyporesponsiveness was chosen. this setting, were associated increased levels plasma metabolites allograft expression enzyme, synthase (iNOS). extent significantly greater rejection. Using situ hybridization, iNOS mRNA localized infiltrating inflammatory cells hepatocytes context contrast, isolated hyporesponsive state. To specifically hepatocyte-derived ablated resulted significant elevation serum transaminase values accompanying histologic evidence periportal infiltration. Our that site production varies according status allograft, may be protective