Determination of free isomeric oleanolic acid and ursolic acid in Pterocephalus hookeri by capillary zone electrophoresis.

摘要: Abstract A rapid capillary zone electophoresis (CZE) method for the quantification of two bioactive terpenes in Pterocephalus hookeri was developed. With β-cyclodextrin as an additive, excellent resolution these hydrophobic isomers can be obtained less than 11 min. Linear calibration range oleanolic acid between 15.6 and 1000 μg/ml ( r  = 0.9998), ursolic 31.2  = 0.9992). The limits detection were 3.4 3.8 μg/ml, respectively. contents free P. determined with recoveries ranging from 95.2 to 106.0%. acids found 0.21 mg/g (RSD 5.49%) 0.53 mg/g 3.37%), After hydrolysis by acid, values 1.12 mg/g 3.29%) 0.43 mg/g 3.42%).