Gene-Silencing-Induced Changes in Carbohydrate Conformation in Relation to Bioenergy Value and Carbohydrate Subfractions in Modeled Plant (Medicago sativa) with Down-Regulation of HB12 and TT8 Transcription Factors

摘要: Gene silencing with RNA interference (RNAi) technology may be capable of modifying internal structure at a molecular level. This structural modification could affect biofunctions in terms biodegradation, biochemical metabolism, and bioactive compound availability. The objectives this study were to (1) Detect gene silencing-induced changes carbohydrate an alfalfa forage (Medicago sativa spp. sativa: alfalfa) down-regulation genes that encode transcription factors TT8 HB12; (2) Determine nutrient bioutilization bioavailability the sativa); (3) Quantify correlation between animals ruminants. experimental treatments included: T1 = Non-transgenic no-gene silenced (code “NT”); T2 HB12-RNAi HB12 down regulation “HB12”); T3 TT8-RNAi “TT8”). determined by non-invasive non-destructive advanced spectroscopy middle infrared radiation region focused on structural, non-structural total compounds. modified using NRC-2001 system digestive (TDN), truly digestible fiber (tdNDF), non-fiber fatty acid (tdFA), crude protein (tdCP) bioenergy profiles (digestible energy, metabolizable net energy) for subfractions evaluated updated CNCPS 6.0 system. results showed significantly affected tdNFC (42.3 (NT) vs. 38.7 (HB12) 37.4% Dry Matter (TT8); p 0.016) tdCP (20.8 19.4 22.3% DM 0.009). gene-silencing also CA4 (7.4 4.2 4.4% (CHO) (TT8), 0.063) CB1 fractions (5.3 2.0 2.6% CHO 0.006). functional group peak area intensity ca. 1315 cm−1 was correlated TDN1x (r −0.83, 0.042) 0.042), height 1370 tdNDF −0.87, 0.025). A_Non-stCHO A_StCHO ratio A_CHO tdFA 0.83–0.91, < 0.05). As fractions, both spectral H_1415 H_1315 (p 0.039; 0.059, respectively), CB3 tended correlate H_1150, H_1100 H_1025 0.10). In conclusion, RNAi-mediated not only inherent but biofunctions. induced RNAi associated digestion.