Characterizing the molecular structure features of newly developed hulless barley cultivars with altered carbohydrate traits (Hordeum vulgare L.) by globar-sourced infrared spectroscopy in relation to nutrient utilization and availability

摘要: Abstract Four newly developed huless barley cultivars with altered carbohydrate traits were at the Crop Development Centre (CDC), University of Saskatchewan which varied for amylose (1–40% DM), and β-glucan (5–10% DM) content. The four hulless include zero-amylose waxy, CDC Fibar; 5%-amylose Rattan; normal-amylose, McGwire high-amylose, HB08302. Copeland was also included as a hulled control. Our previous study revealed carbohydrate traits in affected nutrition availability rumen degradation kinetics. These biological differences may be caused by differences their molecular structure features. objectives this to (1) reveal features traits, (2) quantify structural relation kinetics, intestinal nutrient digestion predicted protein supply dairy cattle. Conventional Molecular Infrared-vibration Spectroscopy applied detect biochemical characteristics functional group bands including protein, non-starch (β-glucan cellulosic compounds) total carbohydrate. Spectral data further analyzed using univariate analysis recording absorption peak parameters (baseline, region, relative height area) multivariate agglomerative hierarchical cluster principal components analysis. Although correlation results showed weak (P