Interleukin 11 expression in the normal canine eye

摘要: Objectives The purpose of this study was to characterize the expression interleukin-11 (IL-11), a cytokine with anti-inflammatory, cytoprotective, and immune-modulating characteristics, in canine eye. Procedures Normal eyes were collected from clinically healthy dogs that had been euthanized for reasons unrelated study. distribution IL-11 different ocular layers evaluated by immunofluorescence (eight eyes). Expression levels quantified (based on fluorescence intensity) using pixel density analysis. Primary cell cultures derived all three corneal layers. mRNA assessed these quantitative RT-PCR before after treatment TGF-b1, known inducer expression. protein also media cells Western blot Results detected epithelium, keratocytes, endothelium normal examined immunofluorescence. Baseline noted fibroblasts, RT-PCR. Treatment lines TGF-b1 resulted statistically significant increases endothelial fibroblast strongest induction fibroblasts endothelium. Conclusion This is first description appear be constitutively present throughout cornea are increased important inflammation disease.