Low levels of tissue factor are compatible with development and hemostasis in mice.

摘要: Tissue factor (TF) expression is associated with life-threatening thrombosis in a variety of human diseases, including sepsis, cancer, and atherosclerosis. Recently, it was shown that inactivation the murine TF (mTF) gene results embryonic lethality. To date, despite extensive studies on regulation promoter vitro, no have examined cis-acting regulatory elements control vivo. Here we report (hTF) minigene containing cDNA directed low level (approximately 1% relative to mouse TF) both constitutive LPS-inducible transgenic mice. Importantly, rescued lethality null embryos, suggesting substituted for during embryogenesis. Rescued mice (mTF-/-, hTF+), which expressed levels 1%) activity, developed normally signs bleeding diathesis, can maintain hemostasis compatible normal survival. These establish novel model system be used examine vivo impact hemostatic balance various thrombotic diseases.