A real-time PCR quantitative detection assay for Pseudomonas savastanoi pv. nerii in Nerium oleander

摘要: A real-time PCR assay based on TaqMan chemistry was developed for the detection of Pseudomonas savastanoi pathovars that cause bacterial knot disease different plant species. Primers and probe sequences were iaaL gene coding (indole-3-acetyl)-L-lysine synthetase previously used in conventional tests. Assay specificity tested with an extended range strains P. from eight hosts, 13 other spp., microorganisms naturally occurring or oleander plants. pure culture cell suspension quantifi ed over a seven log concentration (108 to 102 cfu ml-1 ). Different protocols cation pv. nerii symptomatic asymptomatic 24-h enrichment step either PVF-1 OKA-M broth improved sensitivity assay, making it suitable screen planting material latent infections.