Structure of nucleohistone. III. Interaction with toluidine blue
作者： Akiko Miura , Yoshiki Ohba
DOI: 10.1016/0005-2787(67)90062-7
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摘要: Abstract The interaction of toluidine blue with calf-thymus nucleohistone and DNA was investigated spectrophotometrically special reference to the spectral transition occurring on formation complex. dye found bind in at least two ways (Process I, II). These binding sites could be estimated separately. It by Millipore filtration methods that there were 0.35 0.32 per unit nucleotide respectively for Process 1.00 0.48 II. results indicate a molecule about half phosphate groups are free from basic histones.
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