Effect of early introduction of microencapsulated diet to larval Atlantic halibut, Hippoglossus hippoglossus L. assessed by microarray analysis.

摘要: An experimental microdiet prepared using an internal gelation method was used to partially replace the traditional live feed (Artemia) for larval Atlantic halibut, Hippoglossus hippoglossus L. Three trials were conducted with introduced at 20, 32, and 43 days post first feeding larvae sampled approximately 2, 13, 23, 33 after introduction in each trial. The success of assessed by morphometrics histological analysis gut contents. Microdiet particles readily consumed a period adaptation provided adequate source nutrients no significant increase mortality microdiet-fed group compared control group. However, growth limited there increased incidence malpigmentation eye skin. Subtle changes underlying digestive developmental physiology revealed microarray RNA from fish given day 20 feeding. Fifty-eight genes differentially expressed over four sampling times course trial 28 annotated functions fell into five major categories: metabolism biosynthesis, cell division proliferation, protein trafficking, structure, stress. Interestingly, several these involved pigmentation development, agreement phenotypic abnormalities seen larvae.