Tomato Mosaic Virus Replication Protein Suppresses Virus-Targeted Posttranscriptional Gene Silencing
作者： Kenji Kubota , Shinya Tsuda , Atsushi Tamai , Tetsuo Meshi
DOI: 10.1128/JVI.77.20.11016-11026.2003
关键词:
摘要: Posttranscriptional gene silencing (PTGS), a homology-dependent RNA degradation system, has role in defending against virus infection plants, but plant viruses encode suppressor to combat PTGS. Using transgenic tobacco which the expression of green fluorescent protein (GFP) is posttranscriptionally silenced, we investigated tomato mosaic (ToMV)-encoded PTGS suppressor. Infection with wild-type ToMV (L strain) interrupted GFP tobacco, coincident visible symptoms, whereas some attenuated strains 11 and L A strains) failed suppress silencing. Analyses recombinant containing revealed that single base change replicase gene, causes an amino acid substitution, responsible for symptomless suppressor-defective phenotypes strains. An agroinfiltration assay indicated 130K replication acts as Small interfering RNAs (siRNAs) 21 25 nucleotides accumulated during infection, suggesting major target ToMV-encoded downstream from production siRNAs pathway. Analysis GFP-tagged inhibits establishment ToMV-targeted system inoculated leaves does not detectably activity preexisting, sequence-specific machinery there. Taken together, these results indicate it likely suppressor, protein, blocks utilization silencing-associated small RNAs, so newly formed.
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