Chemotherapy plus G‐CSF mobilized peripheral blood stem cell harvests from acute myeloid leukaemia patients contain large amounts of polyclonal myeloid linnegCD11cpos dendritic precursor cells

摘要: Dendritic cells (DC) are potent antigen-presenting that can induce effective tumour-specific T-cell responses. This study investigated leucapheresis products as source of DC precursors in 48 patients undergoing autologous peripheral blood stem cell (PBSC) transplantation for haematological malignancies. Strikingly, high-dose cytarabine and etoposide plus granulocyte colony stimulating factor (G-CSF) mobilized PBSC harvests from acute myeloid leukaemia (AML) containing the highest number lin(neg)CD11c(pos) (mean: 7.04 x 106/kg, range: 1.46-19.67) which was 18.1-fold higher than non-AML using chemotherapy (CT) regimens G-CSF. Clonality purified CT G-CSF AML (n = 8 ) assessed human androgen-receptor locus methylation, disclosing a polyclonal pattern five female patients. These displayed morphological phenotypic features with expression HLA-DR, HLA-ABC, CD86, CCR5 CD54 molecules but lacking CD80, CD83, CD1a CD40 antigens. Short-term culture leukaemic lysates tumour necrosis factor-alpha yielded maturated capable triggering interleukin-2 interferon-gamma production by T-lymphocytes. findings suggest use post-remission mobilizing regimen generates high doses precursors, could be used to design feasible immunotherapy protocols.