Characterization of a spontaneously generated murine retinal pigmented epithelium cell line; a model for in vitro experiments.

摘要: Abstract Retinal pigmented epithelium (RPE), the outermost layer of retina, has a key role in maintaining retinal cells’ functions. Severity culture RPE cells exerted many limitations to both vitro and vivo studies its therapeutic applications. Therefore, establishment cell lines with high proliferative potential can considerably improve study biology. Here we report generation spontaneously immortalized murine line primary mouse culture. Founded colonized were picked up expression progenitor (RPC) markers studied using immunocytochemistry (ICC). Emerged cultured over 35 passages population doubling times different serum concentrations calculated. We also investigated ability for becoming transfected by calcium-phosphate method infected adeno-associated virus serotype 2 (AAV2) flow cytometry. Data showed that cobblestone constituent expressed RPE65, cytokeratin ZO1 moreover several such as Pax6, Sox2, Nestin Chx10. It revealed that, despite cells, newly emerged easily transfectable highly infectable when compared HEK293T cells. Our data indicated pretended RPC-like phenotype simultaneously markers. would be promising model leading on RPC substantially confirmed great plasticity invaluable research studies.