Isolation and characterization of a spontaneously immortalized bovine retinal pigmented epithelial cell line

摘要: The Retinal Pigmented Epithelium (RPE) is juxtaposed with the photoreceptor outer segments of eye. proximity cells a prerequisite for their survival, as they depend on RPE to remove and are also influenced by cell paracrine factors. death can cause progressive loss function, which diminish vision and, over time, blindness ensues. Degeneration retina has been shown induce variety retinopathies, such Stargardt's disease, Cone-Rod Dystrophy (CRD), Retinitis Pigmentosa (RP), Fundus Flavimaculatus (FFM), Best's disease Age-related Macular (AMD). We have cultured primary bovine gain further understanding mechanisms death. One cultures, named tRPE, surpassed senescence was characterized determine its viability model retinal diseases. tRPE line passaged up 150 population doublings be morphologically similar cells. They origin reverse transcriptase PCR immunocytochemistry using RPE-specific genes RPE65 CRALBP proteins Bestrophin. immunoreactive vimentin, cytokeratin zonula occludens-1 antibodies. Chromosome analysis indicates normal diploid number. do not grow in suspension or soft agar. After 3H thymidine incorporation, appear divide appreciably after confluency. immortal, but still exhibit contact inhibition, serum dependence, monolayer growth secrete an extra-cellular matrix. retain in-vivo morphology, gene expression polarity. Additionally, endocytose exogenous melanin, A2E purified lipofuscin granules. This may useful in-vitro research maculopathies.