Collagen production by cultured human retinal pigment epithelial cells.

摘要: We investigated extracellular matrix produced by human retinal pigment epithelial cells (RPE) in vitro using electron microscopy and enzyme linked immunosorbent assay (ELISA). The thickness of the under cell layer was about 30 microns after 360 days culture. It consisted mainly fibrous granular components. Type IV V collagen were detected but type I III not ELISA. appeared that RPE can secrete form a thick membrane which may cause proliferative vitreoretinopathy (PVR) contraction. Control proliferation secretion is indispensable for prevention PVR.