Functional characterisation of osteosarcoma cell lines and identification of mRNAs and miRNAs associated with aggressive cancer phenotypes

摘要: Osteosarcoma is the most common primary malignant bone tumour, predominantly affecting children and adolescents. Cancer cell line models are required to understand underlying mechanisms of tumour progression for preclinical investigations. To identify lines that well suited studies critical cancer-related phenotypes, such as initiation, growth metastasis, we have evaluated 22 osteosarcoma in vivo tumorigenicity, vitro colony-forming ability, invasive/migratory potential proliferation capacity. Importantly, also identified mRNA microRNA (miRNA) gene expression patterns associated with these phenotypes by profiling. The exhibited a wide range from rather indolent very aggressive. Several mRNAs were differentially expressed highly aggressive compared non-aggressive lines, including RUNX2, several S100 genes, collagen genes encoding proteins involved factor binding, adhesion extracellular matrix remodelling. Most notably, four genes—COL1A2, KYNU, ACTG2 NPPB—were high all investigated, suggesting they might important roles process tumorigenesis. At miRNA level, miR-199b-5p mir-100-3p downregulated whereas miR-155-5p, miR-135b-5p miR-146a-5p upregulated. further predicted be linked metastatic capacity disease. detailed characterisation will support selection use specific miRNAs this study may represent good candidates future therapeutic targets. our knowledge, first time profiles functional characteristics lines.