Quantification of Proteins with a New Sensitive Method — Zone Immunoelectrophoresis Assay

摘要: Quantitative determination of proteins has been made by electrophoresis thereof in tubular canals filled with agarose gel containing antibodies. The procedure is simple and practicable. Sample volumes a few microliter up to some hundred may be added for each test. amount protein sample was found linearly related the distance from point application (i.e. surface) front immunoprecipitation zone. zone immunoelectrophoresis method shows over rocket following advantages: less consumption antibodies; much lower detection limit (0.05 microgram per ml); larger concentration range linear calibration curve. A high correlation (0.98) between first- last-mentioned obtained alpha 1-antitrypsin. Precision data are given albumin alpha-fetoprotein typically relative standard deviation percent obtained.