Transcription coactivator TRAP220 is required for PPARγ2-stimulated adipogenesis
作者： Kai Ge , Mohamed Guermah , Chao-Xing Yuan , Mitsuhiro Ito , Annika E. Wallberg
DOI: 10.1038/417563A
关键词:
摘要: The TRAP (thyroid hormone receptor-associated proteins) transcription coactivator complex (also known as Mediator) was first isolated a group of proteins that facilitate the function thyroid receptor1. This interacts physically with several nuclear receptors through TRAP220 subunit, and diverse activators other subunits2. has been reported to show ligand-enhanced interaction peroxisome proliferator-activated receptor γ2 (PPARγ2)3,4, essential for adipogenesis5,6,7,8. Here we Trap220-/- fibroblasts are refractory PPARγ2-stimulated adipogenesis, but not MyoD-stimulated myogenesis, do express adipogenesis markers or PPARγ2 target genes. These defects can be restored by expression exogenous TRAP220. Further indicative direct role in via complex, functions directly transcriptional purified vitro system ligand- TRAP220-dependent manner. data indicate acts, PPARγ2-selective and, accordingly, it is specific one fibroblast differentiation pathway (adipogenesis) relative another (myogenesis).
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Christophe Rachez, Bryan D. Lemon, Zalman Suldan, Virginia Bromleigh, Matthew Gamble, Anders M. Näär, Hediye Erdjument-Bromage, Paul Tempst, Leonard P. Freedman, Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. Nature. ,vol. 398, pp. 824- 828 ,(1999) , 10.1038/19783
Thomas G. Boyer, Michelle E. D. Martin, Emma Lees, Robert P. Ricciardi, Arnold J. Berk, Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein Nature. ,vol. 399, pp. 276- 279 ,(1999) , 10.1038/20466
Anders M. Näär, Pierre A. Beaurang, Sharleen Zhou, Shaji Abraham, William Solomon, Robert Tjian, Composite co-activator ARC mediates chromatin-directed transcriptional activation Nature. ,vol. 398, pp. 828- 832 ,(1999) , 10.1038/19789
Yaacov Barak, Michael C Nelson, Estelita S Ong, Ying Z Jones, Pilar Ruiz-Lozano, Kenneth R Chien, Alan Koder, Ronald M Evans, PPARγ Is Required for Placental, Cardiac, and Adipose Tissue Development Molecular Cell. ,vol. 4, pp. 585- 595 ,(1999) , 10.1016/S1097-2765(00)80209-9
Bradford B Lowell, PPARgamma: an essential regulator of adipogenesis and modulator of fat cell function. Cell. ,vol. 99, pp. 239- 242 ,(1999) , 10.1016/S0092-8674(00)81654-2
Xiaoqing Sun, Yi Zhang, Helen Cho, Paula Rickert, Emma Lees, William Lane, Danny Reinberg, NAT, a Human Complex Containing Srb Polypeptides that Functions as a Negative Regulator of Activated Transcription Molecular Cell. ,vol. 2, pp. 213- 222 ,(1998) , 10.1016/S1097-2765(00)80131-8
Zhidan Wu, Evan D Rosen, Regina Brun, Stefanie Hauser, Guillaume Adelmant, Amy E Troy, Catherine McKeon, Gretchen J Darlington, Bruce M Spiegelman, Cross-Regulation of C/EBPα and PPARγ Controls the Transcriptional Pathway of Adipogenesis and Insulin Sensitivity Molecular Cell. ,vol. 3, pp. 151- 158 ,(1999) , 10.1016/S1097-2765(00)80306-8
Mitsuhiro Ito, Chao-Xing Yuan, Hirotaka J Okano, Robert B Darnell, Robert G Roeder, Involvement of the TRAP220 Component of the TRAP/SMCC Coactivator Complex in Embryonic Development and Thyroid Hormone Action Molecular Cell. ,vol. 5, pp. 683- 693 ,(2000) , 10.1016/S1097-2765(00)80247-6
Kai Ge, James DuHadaway, Wei Du, Meenhard Herlyn, Ulrich Rodeck, George C Prendergast, Mechanism for elimination of a tumor suppressor: aberrant splicing of a brain-specific exon causes loss of function of Bin1 in melanoma. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 9689- 9694 ,(1999) , 10.1073/PNAS.96.17.9689
Peter Tontonoz, Erding Hu, Bruce M. Spiegelman, Stimulation of adipogenesis in fibroblasts by PPARγ2, a lipid-activated transcription factor Cell. ,vol. 79, pp. 1147- 1156 ,(1994) , 10.1016/0092-8674(94)90006-X