Human class I alcohol dehydrogenases catalyze the interconversion of alcohols and aldehydes in the metabolism of dopamine.
作者： Goeran Maardh , Bert L. Vallee
DOI: 10.1021/BI00371A005
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摘要: The class I human liver alcohol dehydrogenases (ADHs) catalyze the interconversion of intermediary alcohols and aldehydes dopamine metabolism in vitro, whereas those II III do not. individual, homogeneous isozymes oxidize (3,4-dihydroxyphenyl)ethanol (4-hydroxy-3-methoxyphenyl)ethanol (HMPE) ethanol with kcat/Km values range from 16 to 240 mM-1 min-1 66 min-1, respectively. They reduce corresponding (3,4-dihydroxyphenyl)acetaldehyde (4-hydroxy-3-methoxyphenyl)acetaldehyde (HMPAL) varying 7800 190,000 considerably more efficient than reduction acetaldehyde 780 4900 min-1. For beta 1 gamma 2 ADH, competes HMPE oxidation a Ki 23 microM. In addition, 1,10-phenanthroline inhibits HMPAL 20 microM 12 microM, respectively, both quite similar that for ethanol, = 22 Thus, ethanol/acetaldehyde intermediates compete same site basis ethanol-induced vivo alterations metabolism.
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sci-hub.se 参考文章(19)
V. Gene Erwin, Richard A. Deitrich, Brain aldehyde dehydrogenase. Localization, purification, and properties. Journal of Biological Chemistry. ,vol. 241, pp. 3533- 3539 ,(1966) , 10.1016/S0021-9258(18)99864-9
Jean-Pierre von Wartburg, Bendicht Wermuth, [85] Aldehyde reductase from human tissues Methods in Enzymology. ,vol. 89, pp. 506- 513 ,(1982) , 10.1016/S0076-6879(82)89087-3
T.Geoffrey Flynn, Aldehyde reductases: Monomeric NAPDH-dependent oxidoreductases with multifunctional potential Biochemical Pharmacology. ,vol. 31, pp. 2705- 2712 ,(1982) , 10.1016/0006-2952(82)90123-X
Bendicht Wermuth, Jacqueline D.B. Mu¨nch, Reduction of biogenic aldehydes by aldehyde reductase and alcohol dehydrogenase from human liver. Biochemical Pharmacology. ,vol. 28, pp. 1431- 1433 ,(1979) , 10.1016/0006-2952(79)90449-0
A.William Tank, Henry Weiner, Ethanol-induced alteration of dopamine metabolism in rat liver. Biochemical Pharmacology. ,vol. 28, pp. 3139- 3147 ,(1979) , 10.1016/0006-2952(79)90624-5
Göran Mårdh, Birgitta Sjöquist, Erik ÄnggÅrd, Norepinephrine Metabolism in Man Using Deuterium Labelling: The Conversion of 4-Hydroxy-3-Methoxyphenylglycol to 4-Hydroxy-3-Methoxymandelic Acid Journal of Neurochemistry. ,vol. 36, pp. 1181- 1185 ,(1981) , 10.1111/J.1471-4159.1981.TB01716.X
G. Mardh, C. A. Luehr, B. L. Vallee, Human class I alcohol dehydrogenases catalyze the oxidation of glycols in the metabolism of norepinephrine Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 4979- 4982 ,(1985) , 10.1073/PNAS.82.15.4979
W. A. Frey, B. L. Vallee, Digitalis metabolism and human liver alcohol dehydrogenase Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 924- 927 ,(1980) , 10.1073/PNAS.77.2.924
Charles C. Ditlow, Barton Holmquist, Maurice M. Morelock, Bert L. Vallee, Physical and enzymatic properties of a class II alcohol dehydrogenase isozyme of human liver: pi-ADH. Biochemistry. ,vol. 23, pp. 6363- 6368 ,(1984) , 10.1021/BI00321A012
George Waller, Hugo Theorell, Jan Sjövall, Liver alcohol dehydrogenase as a 3β-hydroxy-5β-cholanic acid dehydrogenase Archives of Biochemistry and Biophysics. ,vol. 111, pp. 671- 684 ,(1965) , 10.1016/0003-9861(65)90249-3