Diagnostic potential ofsefADNA probes toSalmonella enteritidisand certain other O-serogroup D1Salmonellaserovars

摘要: Abstract Salmonella enteritidis thin fimbriae, SEF14, were found to be restricted S. dublin and the predominantly poultry-associated members of O-serogroup D1, , berta gallinarum pullorum when tested by Western ELISA analyses from among 90 isolates 42 serovars, as well several related genera Enterobacteriaceae . These five serovars a single isolate typhi (D1) also detected hybridization genomic DNA 732 117 serogroups gene probes derived sefA (fimbrin gene), sefB (chaperone) or sefC (outer membrane protein) genes encoding proteins involved in SEF14 biosynthesis. None 250 27 other eubacterial hybridized sef probes. The amplified these six PCR using primer pairs designed sequencing indicated limited sequence variability recognition individual base which could potentially differentiate certain strains