Detection of Salmonella enteritidis by reverse transcription-polymerase chain reaction (PCR)

摘要: A reverse transcription-polymerase chain reaction (RT-PCR) method was developed for detecting mRNA from the sefA gene of Salmonella enteritidis. Detection target examined cells grown in buffered peptone water at different temperatures (37, 25 and 15°C) pH (5.5, 7.2 8.5). The results revealed that levels transcription differed depending upon physiological state cells. This affected sensitivity RT-PCR assay. When assay evaluated detection S. enteritidis PT4 artificially contaminated minced beef whole egg samples, an enrichment step used (buffered water, 7.2, 37°C, 16 h) to increase In presence normal background flora each food type, it possible detect ten after a 16-h using assay, with total testing time 28 h. Unlike PCR test tested parallel, did not nonviable (heat-inactivated) supported usefulness as viable microorganisms.