Rapid, specific detection of Salmonella Enteritidis in pooled eggs by real-time PCR.

作者: K. H. SEO , I. E. VALENTIN-BON , R. E. BRACKETT , P. S. HOLT

DOI: 10.4315/0362-028X-67.5.864

关键词: NucleaseBiologyBacteriaSalmonellaTaqManPolymerase chain reactionReal-time polymerase chain reactionEnterobacteriaceaeSalmonella enteritidisMicrobiology

摘要: … to Salmonella serogroup D strains such as Gallinarum, … PCR reaction mix consisting of TaqMan Universal PCR Master Mix (25 ml), forward primer (5 ml), reverse primer (5 ml), TaqMan …

参考文章(21)
PETER S. HOLT, RICHARD K. GAST, CAM R. GREENE, Rapid Detection of Salmonella enteritidis in Pooled Liquid Egg Samples Using a Magnetic Bead-ELISA System. Journal of Food Protection. ,vol. 58, pp. 967- 972 ,(1995) , 10.4315/0362-028X-58.9.967
Sakchai Himathongkham, Hans Riemann, Ralph Ernst, Efficacy of disinfection of shell eggs externally contaminated with Salmonella enteritidis: Implications for egg testing International Journal of Food Microbiology. ,vol. 49, pp. 161- 167 ,(1999) , 10.1016/S0168-1605(99)00092-6
JOHN L. McKILLIP, MARYANNE DRAKE, Molecular beacon polymerase chain reaction detection of Escherichia coli O157:H7 in milk. Journal of Food Protection. ,vol. 63, pp. 855- 859 ,(2000) , 10.4315/0362-028X-63.7.855
Elizabeth A Szabo, Bernard M Mackey, Detection of Salmonella enteritidis by reverse transcription-polymerase chain reaction (PCR) International Journal of Food Microbiology. ,vol. 51, pp. 113- 122 ,(1999) , 10.1016/S0168-1605(99)00106-3
M. J. Woodward, I. Mclaren, C. Wray, Distribution of virulence plasmids within Salmonellae. Microbiology. ,vol. 135, pp. 503- 511 ,(1989) , 10.1099/00221287-135-3-503
Ingeborg Hein, Dieter Klein, Angelika Lehner, Andreas Bubert, Ernst Brandl, Martin Wagner, Detection and quantification of the iap gene of Listeria monocytogenes and Listeria innocua by a new real-time quantitative PCR assay Research in Microbiology. ,vol. 152, pp. 37- 46 ,(2001) , 10.1016/S0923-2508(00)01166-9
T.J. Humphrey, Contamination of egg shell and contents with Salmonella enteritidis: a review. International Journal of Food Microbiology. ,vol. 21, pp. 31- 40 ,(1994) , 10.1016/0168-1605(94)90197-X
K. A. Lampel, S. P. Keasler, D. E. Hanes, Specific detection of Salmonella enterica serotype Enteritidis using the polymerase chain reaction. Epidemiology and Infection. ,vol. 116, pp. 137- 145 ,(1996) , 10.1017/S0950268800052365