Comprehensive profiling of circulating microRNA via small RNA sequencing of cDNA libraries reveals biomarker potential and limitations
作者： Zev Williams , Iddo Z. Ben-Dov , Rony Elias , Aleksandra Mihailovic , Miguel Brown
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摘要: We profiled microRNAs (miRNAs) in cell-free serum and plasma samples from human volunteers using deep sequencing of barcoded small RNA cDNA libraries. By introducing calibrator synthetic oligonucleotides during library preparation, we were able to calculate the total as well specific concentrations circulating miRNA. Studying trios newborn babies their parents detected placental-specific miRNA both maternal circulations quantitated relative contribution placental miRNAs pool miRNAs. Furthermore, sequence variation profiles could be traced placenta origin. These profiles, which may serve a model for tumor or disease detection, allow us define repertoire abundance circulation potential uses biomarkers.
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