Self-Compatibility of Two Apricot Selections Is Associated with Two Pollen-Part Mutations of Different Nature

摘要: Loss of pollen-S function in Prunus self-compatible mutants has recently been associated with deletions or insertions S-haplotype-specific F-box (SFB) genes. We have studied two cultivars apricot (Prunus armeniaca), Currot (SCSC) and Canino (S2SC), sharing the naturally occurring (SC)-haplotype. Sequence analysis showed that whereas SC-RNase is unaltered, a 358-bp insertion found SFBC gene, resulting expression truncated protein. The alteration this gene self-incompatibility (SI) breakdown, supporting previous evidence points to SFB being SI S-locus. On other hand, PCR progenies derived from pollen grains carrying S2-haplotype were also able overcome incompatibility barrier. However, alterations SFB2 duplications not detected. A new class genes encoding previously uncharacterized protein high sequence similarity (approximately 62%) proteins was identified work, but available data rules them out producing S-heteroallelic thus cause pollen-part mutation. These results suggest cv an additional mutation, linked S-locus, which causes loss activity when present pollen. As whole, these findings support proposal S-locus products besides independent factors are required for gametophytic Prunus.