Fluorescence in sub-10 nm channels with an optical enhancement layer

摘要: Fluorescence microscopy uniquely enables physical and biological research in micro- nanofluidic systems. However, channels with depths below 10 nm, the limited number of fluorophores results fluorescence intensity detection limit optical microscopes. To overcome this barrier, we applied Fabry–Perot interference to enhance a silicon nitride layer sub-10 nm channel. A suitable thickness can selectively both absorption emission wavelengths, leading fluorescent signal that is enhanced 20-fold readily imaged traditional demonstrate method, studied mass transport binary solution ethanol Rhodamin B 8 nanochannels. The large molecular size (∼1.8 nm) relative channel depth separation reduced diffusivity, deviating from behavior at larger scales. This method extends widely available suite analysis tools infrastructure unprecedented scale relevance wide variety biomolecular interactions.