Long noncoding RNA UCA1 promotes cell growth, migration, and invasion by targeting miR-143-3p in oral squamous cell carcinoma.
作者： Qingyun Duan , Mei Xu , Meng Wu , Xiong Zhang , Min Gan
DOI: 10.1002/CAM4.2808
关键词:
摘要: BACKGROUND The long noncoding RNA (lncRNA) urothelial carcinoma-associated 1 (UCA1) is dysregulated in many types of tumors; however, its role oral squamous cell carcinoma (OSCC) remains unclear. This study aims to determine the effect lncRNA UCA1 on OSCC. METHODS Fifty-six paired OSCC and adjacent nontumorous tissues were collected levels UCA1, miR-143-3p, MYO6 evaluated by qRT-PCR. In vitro experiments, viability, migration, invasion measured by, respectively, performing CCK-8, wound healing, transwell assays. target relationships among verified dual-luciferase assay. Western blot immunohistochemistry carried out protein levels. Xenograft mouse model was established explore effects vivo. RESULTS Levels increased significantly OSCC, while level miR-143-3p decreased compared with tissues. promoted growth, both vivo, reversed progression. validated as a for overexpression mimic cells. CONCLUSION LncRNA contributes proliferation metastasis cells targeting upregulating downstream gene MYO6. could serve promising novel therapy treatment
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