Lentivirus-Mediated Silencing of Myosin VI Inhibits Proliferation and Cell Cycle Progression in Human Lung Cancer Cells.

摘要: Myosin VI (MYO6) is a unique actin motor, which moves toward the pointed ends of filaments. In this study, we found that MYO6 overexpressed in lung cancer tissues and associated with progression, particularly lymph node metastasis. To investigate its functions cells, generated recombinant lentivirus taking shRNA MYO6. Using two cell lines, A549 95D, Lv-shMYO6 could infect cells high efficiency downregulate on both mRNA protein levels. After knockdown MYO6, proliferation rates were decreased significantly. The colony-formation ability MYO6-silenced was also impaired reduced colony numbers fewer per colony. Flow cytometry showed cycle progression stuck at G0 /G1 phase, especially sub-G1 represents apoptotic cells. Moreover, downregulated phosphorylation ERK1/2. Further experiments using another confirmed above results. These results suggest crucial maintaining growth may serve as potential therapeutic target for treatment.