Correction of abnormal lipid fluidity and composition of rat ileal microvillus membranes in chronic streptozotocin-induced diabetes by insulin therapy.
作者： T A Brasitus , P K Dudeja
DOI: 10.1016/S0021-9258(17)38888-9
关键词:
摘要: Diabetes was induced in rats by administration of streptozotocin. After 90-120 days, one group chronic diabetic animals treated with insulin for 10 days. The lipid fluidity and composition microvillus membranes prepared from ileal enterocytes control, diabetic, insulin-treated were determined. Lipid fluidity, as assessed steady-state fluorescence polarization techniques using the probes 1,6-diphenyl-1,3,5-hexatriene, DL-2-(9-anthroyl)stearic acid DL-12-(9-anthroyl)stearic acid, decreased compared to control membranes. differences resulted an increased cholesterol content cholesterol/phospholipid molar ratio animals. activities sucrase alkaline phosphatase also found be higher Insulin treatment, however, failed significantly influence enzymatic these These studies, therefore, demonstrate that alterations composition, certain exist streptozotocin-induced rats. Administration days restored membrane but not levels.
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