Studies on activation of T4 bacteriophage by cofactor I. The degree of activity

作者: Elie L. Wollman , Gunther S. Stent

DOI: 10.1016/0006-3002(50)90103-X

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摘要: Resume 1. De la relation existant entre vitesse initiale d'adsorption du bacteriophage T4, qui a besoin de confacteur pour etre adsorbe par les bacteries sensibles, et concentration tryptophane dans le milieu synthetique, est tiree notion degre d'activite, defini comme etant rapport une cofacteur donnee maxima observee. 2. Apres dilution brusque d'un melange suspension bacterienne, deux phenomenes se produisent concuremment: perte d'activite adsorption sur bacteries. Cette methode competitive permet restreindre peut considere propriete caracteristique population bacteriophages, independante des conditions 3. Le lysat bacteriophagique utilise nos experiences homogene en ce concerne son activabilite. represente donc statistique bacteriophages. 4. En utilisant concentrations elevees telles qu'ils entrent competition present, on estimer l'ordre grandeur nombre molecules fixees corpuscule bacteriophage. Ce d'environ 300 (limite superieure) activite correspondant 1 3 l'activite maxima. 5. Deux conceptions — celle tout-ou-rien etats intermediaire—ayant but rapporter au corpus cule sont considerees.

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