Ultrastructure of Cochliobolus victoriae conidia incubated on fungistatic soil

摘要: When incubated on soil, conidiospores of the soil-borne fungus Cochliobolus victoriae remain viable but do not germinate, a phenomenon termed soil fungistasis. We examined these spores ultrastructurally for signs dormancy or metabolic activity. The was cultured media containing pyroquilon (1 g ml −1 ), an inhibitor melanin biosynthesis, in order to facilitate efficient fixation. > 1 pyroquilon, had reduced sensitivity At growth medium, no significant effect conidia fungistasis, germination rate non-fungistatic conditions, concentration SDS-soluble protein conidia, and sporulation colonies. In 0–96 h, nucleoli were prominent, endoplasmic reticulum well developed, mitochondria well-developed cristae. During incubation there appeared be decrease unidentified electron-translucent material cytoplasm. ultrastructure suggests that, at least this initial 96 h period, non-germinating fungistatic are metabolically active, dormant.