Enhanced suppression of adenovirus replication by triple combination of anti-adenoviral siRNAs, soluble adenovirus receptor trap sCAR-Fc and cidofovir.
作者： Tanja Pozzuto , Carsten Röger , Jens Kurreck , Henry Fechner
DOI: 10.1016/J.ANTIVIRAL.2015.05.010
关键词:
摘要: Abstract Adenoviruses (Ad) generally induce mild self-limiting respiratory or intestinal infections but can also cause serious disease with fatal outcomes in immunosuppressed patients. Antiviral drug therapy is an important treatment for adenoviral its efficiency limited. Recently, we have shown that gene silencing by RNA interference (RNAi) a promising new approach to inhibit infection. In the present vitro study, examined whether of RNAi-based anti-adenoviral be further increased combination virus receptor trap sCAR-Fc and antiviral cidofovir. Initially, three siRNAs, siE1A_4, siIVa2_2 Pol-si2, targeting E1A, IVa2 DNA polymerase mRNAs, respectively, were used silencing. Replication Ad was inhibited dose dependent manner each siRNA, inhibition differed (Pol-si2 > siIVa2_2 > siE1A_4). Double triple combinations siRNAs compared single did not result measurably higher suppression replication. Combination (alone mixes two siRNAs) markedly replication same siRNA without sCAR-Fc. Moreover, mix all cidofovir about 23-fold more efficient than mix/sCAR-Fc 95-fold alone. These data demonstrate co-treatment cells suitable increase siRNAs.
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