Growth and differentiation in vitro of mast cells from mesenteric lymph nodes of Nippostrongylus brasiliensis-infected rats.

摘要: Intestinal mastocytosis begins to develop in rats, depending on the strain, at 14 (outbred Sprague-Dawley, SD) or 16 (inbred Lewis, L) days after infection with nematode Nippostrongylus brasiliensis (Nippo). We have investigated vitro mastopoiesis from mesenteric lymph node (MLN) cells cultured various intervals post-infection, using a modified Marbrook liquid system. Greater increases mast (MC) were observed cultures of SD-MLN removed day Nippo (IMLN-14) than MLN uninfected animals (NMLN): seven- twenty-fold versus up two-fold 2 weeks and forty- two hundred-fold 4 weeks, respectively (P < 0.002). In contrast, similar differential MC histamine compared controls, demonstrated week L strain rats 17 (IMLN-17) 20 (IMLN-20) but not 0.001). presence phytohaemagglutinin (PHA) was associated enhanced differentiation both IMLN-17 IMLN-20, while worm antigen (Ag) stimulated IMLN-17, suppressed response IMLN-20 0.02). Conditioned media (CM) prepared unstimulated PHA-stimulated IMLN-32 (i.e. 32 infection) caused significant NMLN when no CM Ag-stimulated 0.01). Either precursors which help exist increased numbers Nippo-infected rats. Mitogenic antigenic stimulation modulates mastopoiesis, either directly through soluble factors derivable cells. These methods can be utilized understand further mechanisms intestinal rat.