Copper exposure modifies the content and distribution of trace metals in mammalian cultured cells

作者: Lucía Tapia , Miriam Suazo , Christian Hödar , Verónica Cambiazo , Mauricio González

DOI: 10.1023/A:1020766932605

关键词:

摘要: With this work, we have determined the cellular content of Cu, Fe and Zn in different cell lines, by using total reflection X-ray fluorescence spectrometry (TXRF). In addition, examined whether exposure to 100 μmoles l−1 Cu-His modifies intracellular distribution these trace metals. Our results indicate that all lines displayed same pattern relative abundance metals (Cu

参考文章(33)
Kwo-Yih Yeh, Mary Yeh, J. Abra Watkins, Juan Rodriguez-Paris, Jonathan Glass, Dietary iron induces rapid changes in rat intestinal divalent metal transporter expression. American Journal of Physiology-gastrointestinal and Liver Physiology. ,vol. 279, ,(2000) , 10.1152/AJPGI.2000.279.5.G1070
Hiromi Gunshin, Bryan Mackenzie, Urs V. Berger, Yoshimi Gunshin, Michael F. Romero, Walter F. Boron, Stephan Nussberger, John L. Gollan, Matthias A. Hediger, Cloning and characterization of a mammalian proton-coupled metal-ion transporter Nature. ,vol. 388, pp. 482- 488 ,(1997) , 10.1038/41343
David R. Brown, Kefeng Qin, Jochen W. Herms, Axel Madlung, Jean Manson, Robert Strome, Paul E. Fraser, Theo Kruck, Alex von Bohlen, Walter Schulz-Schaeffer, Armin Giese, David Westaway, Hans Kretzschmar, The cellular prion protein binds copper in vivo Nature. ,vol. 390, pp. 684- 687 ,(1997) , 10.1038/37783
R Uauy, M Olivares, M Gonzalez, Essentiality of copper in humans. The American Journal of Clinical Nutrition. ,vol. 67, ,(1998) , 10.1093/AJCN/67.5.952S
Maryam Hazegh-Azam, Maria C Linder, Copper biochemistry and molecular biology The American Journal of Clinical Nutrition. ,vol. 63, ,(1996) , 10.1093/AJCN/63.5.797
Julian F.B Mercer, The molecular basis of copper-transport diseases. Trends in Molecular Medicine. ,vol. 7, pp. 64- 69 ,(2001) , 10.1016/S1471-4914(01)01920-7
Edward D. Harris, The Iron-Copper Connection: the Link to Ceruloplasmin Grows Stronger Nutrition Reviews. ,vol. 53, pp. 170- 173 ,(2009) , 10.1111/J.1753-4887.1995.TB01545.X
Steven D. Aust, Lee A. Morehouse, Craig E. Thomas, Role of metals in oxygen radical reactions Journal of Free Radicals in Biology & Medicine. ,vol. 1, pp. 3- 25 ,(1985) , 10.1016/0748-5514(85)90025-X