Growth and recombinant protein expression with Escherichia coli in different batch cultivation media

摘要: Parallel operated milliliter-scale stirred tank bioreactors were applied for recombinant protein expression studies in simple batch experiments without pH titration. An enzymatic glucose release system (EnBase), a complex medium, and the frequently used LB TB media compared with regard to growth of Escherichia coli (alcohol dehydrogenase (ADH) from Lactobacillus brevis formate (FDH) Candida boidinii). Dissolved oxygen recorded online, optical densities measured at-line, activities ADH FDH analyzed offline. Best was observed medium maximum dry cell weight concentrations 14 g L(-1). EnBase cultivations enabled final between 6 8 The remained nearly constant due continuous release, showing usefulness this especially pH-sensitive bioprocesses. Cell-specific enzyme varied considerably depending on different used. Maximum specific h after induction IPTG, whereas highest achieved at low profiles 24 induction. Hence, protein, compositions, times induction, harvest have be evaluated achieve efficient proteins E. coli. A rapid experimental evaluation can easily performed parallel small-scale bioreactors.