Recombinant Escherichia coli with sulfide:quinone oxidoreductase and persulfide dioxygenase rapidly oxidises sulfide to sulfite and thiosulfate via a new pathway.

摘要: Summary Many heterotrophic bacteria contain sulfide:quinone oxidoreductase (SQR) and persulfide dioxygenase (PDO) genes. It is unclear how these enzymes cooperate to oxidise sulfide in bacteria. Cupriavidus pinatubonensis JMP134 contains a gene cluster of sqr pdo, their functions were analysed Escherichia coli. Recombinant E. coli cells with SQR PDO rapidly oxidised thiosulfate sulfite. The also DUF442 domain that was shown have rhodanese activities. SQR-C94S, an active site mutant the domain, transitory accumulation polysulfides. Cellular enzymatic evidence showed speeds up reaction polysulfides glutathione produce (GSSH). Thus, oxidises polysulfides; enhances GSSH; GSSH sulfite; sulfite spontaneously reacts generate thiosulfate. pathway different from proposed mitochondrial because it has polysulfides, is, disulfide trisulfide, as intermediates. data demonstrated can sulfite, providing foundation for using bioremediation.