Design, Construction, and In Vitro Analyses of Multivalent Antibodies

摘要: Some Abs are more efficacious after being cross-linked to form dimers or multimers, presumably as a result of binding and clustering surface target either amplify diversify cellular signaling. To improve the therapeutic potency these types Abs, we designed generated that express tandem Fab repeats with aim mimicking Abs. The versatile design system enables creation series multivalent human IgG Ab forms including tetravalent IgG1, F(ab')2, linear multimers three four consecutively linked Fabs. multimerized cell receptors HER2, death receptor 5, CD20, than their parent mAbs in triggering antitumor responses, indicating they could be useful both reagents for study well novel therapeutics.