Changes of FAME profiles as a marker of phenol degradation in different soils inoculated with Pseudomonas sp. CF600

作者: Agnieszka Mrozik , Mariusz Cycoń , Zofia Piotrowska-Seget

DOI: 10.1016/J.IBIOD.2009.11.002

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摘要: Abstract Fatty acid profiles of three different sterile soils contaminated with phenol at the concentrations 1.7, 3.3 and 5.0 mg g−1 inoculated phenol-degrading Pseudomonas sp. CF600 were investigated. The rate biodegradation survival inoculants in also studied. It was found that introduced strain able to completely degrade added highest dose during 108 days MF WM soils, 124 PF soil, whereas smallest within 60 independently soil type. In all phenol-polluted decrease initial inoculum estimated end experiment treated concentration constituted 18, 21 25% number MF, respectively. Under exposure important changes FAMEs obtained directly from detected. most significant alterations observed dosage related 17:0 cy 19:0 ω8c cyclopropane fatty acids. Over time decreasing decline amount observed. turn, polluted appeared first on day 12 when doses declined about 60% 40% soil. Other FAME composition included slight increase straight-chain, branched, hydroxylated unsaturated value sat/unsat ratio, occurrence distribution specific acids might be useful for estimation progress.

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