Effect of curcumin on the modulation of αA- and αB-crystallin and heat shock protein 70 in selenium-induced cataractogenesis in Wistar rat pups

摘要: Purpose: To investigate the expression of αA- and αB-crystallin heat shock protein 70 (Hsp 70) during curcumin treatment selenium-induced cataractogenesis in Wistar rat pups. Methods: Group I pups received only saline served as control. II were intraperitoneally injected with selenium (15 µM/kg bodyweight) to induce cataract. III also underwent cataract but cotreated 75 mg/kg body weight (single oral dose). IV post-treated at group dosage 24 h after administration. V pretreated before Results: This study found higher levels Hsp lenses alone (group II) than control I). Similar results observed lenses. In contrast, V, presence injection decreased almost same those Conclusions: Curcumin suppressed selenite-induced 70, may therefore suppress formation Pathophysiologically, one early signs is damage lens cell membrane [1], apparently due a decline its ability actively transport substances against electrochemical gradients, resulting alterations intraocular composition metabolism. The connection these effects subsequent opacification tissue, characterized by disulfide-linked proteins molecular weights lower solubility [2], quite complex. Such crosslinks are primarily established between glutathione proteins, especially crystallins. Cataract from loss transparency normal crystallin eye lens, it major cause blindness. However, many risk factors associated pathogenesis cataract, among which aging, diabetes, dominant [3-5]. contains high concentration α-crystallin, predominant vertebrate constitutes up 50% total water-soluble fraction. It normally composed two subunits, αB-crystallin, both approximately 20 kDa [6,7]. virtue special structural features concentration,